The Evidence · Human Clinical Trials

Built on proof, not hype.

Every ingredient in Protocol 01 is selected on the strength of published human clinical research. Explore the science behind the formula.

31
Active ingredients
2,000+
Human clinical papers
100%
Clinically dosed actives
0
Synthetic fillers
Figures reflect the published research base across the ingredient categories in Protocol 01.

The Evidence Dashboard

Published human clinical research across every functional category in the formula.

Category Functional ingredients Human clinical papers Outcomes measured Common findings
NAD+ Boosters Nicotinamide Riboside, Vitamin B3 (Nicotinamide) 150+papers NAD+ blood levels, cellular energy metabolism Consistent, dose-dependent elevation of NAD+ in healthy adults over 40
Sirtuin Activators Trans-Resveratrol, Quercetin (Sophora Japonica) 320+papers SIRT1 activity, cardiovascular & metabolic markers Support for longevity signalling pathways and healthy inflammatory response
Mitochondrial Support Coenzyme Q10, Alpha Lipoic Acid, Magnesium 410+papers ATP production, oxidative stress, fatigue Improved mitochondrial efficiency and reduced markers of oxidative stress
Cognitive & Brain Lion's Mane Extract, Ginkgo Biloba 260+papers Memory, focus, nerve growth factor (NGF) Support for cognitive performance and neuroplasticity in adults
Antioxidant Network Astaxanthin, Vitamin C, Selenium, Zinc 550+papers Oxidative stress, skin, immune function Robust antioxidant activity and support for immune and skin health
Structural & Joint Creatine, Glycine, Glucosamine, Hyaluronic Acid 480+papers Strength, joint comfort, collagen synthesis Well-established support for muscle, joint and connective tissue health
Methylation & DNA Repair Betaine Anhydrous, L-5-MTHF Folate, Vitamin B12 230+papers Homocysteine, methylation status, DNA repair Support for healthy methylation cycles and cardiovascular markers

The Research Library

Nicotinamide Riboside

Chronic nicotinamide riboside supplementation increases NAD+ and is well tolerated in healthy middle-aged and older adults

201824 participantsPlacebo controlledPeer reviewed Read Study Nicotinamide Riboside

A randomised placebo-controlled trial of NR on NAD+ metabolome in healthy adults

2019140 participantsPlacebo controlledPeer reviewed Read Study Trans-Resveratrol

Resveratrol supplementation and cardiovascular risk factors: a meta-analysis of randomised controlled trials

2015Meta-analysisPeer reviewed Read Study Coenzyme Q10

Coenzyme Q10 supplementation and exercise-induced fatigue: a randomised, double-blind trial

200822 participantsPlacebo controlledPeer reviewed Read Study Lion's Mane

Improving effects of Hericium erinaceus (Lion's Mane) on mild cognitive impairment: a double-blind placebo-controlled trial

200930 participantsPlacebo controlledPeer reviewed Read Study Alpha Lipoic Acid

Alpha-lipoic acid as a dietary supplement: molecular mechanisms and therapeutic potential

2009ReviewPeer reviewed Read Study Astaxanthin

Astaxanthin supplementation and oxidative stress, immune response in healthy adults

201042 participantsPlacebo controlledPeer reviewed Read Study Creatine

Creatine supplementation with specific view to exercise, sports performance and ageing

2017Position standPeer reviewed Read Study
Showing 8 of 12 studies

Studies referenced examine individual ingredients, not Protocol 01 itself. Results relate to the specific doses, populations and durations studied. This information is educational and does not constitute health claims about our product. Food supplements are not a substitute for a varied, balanced diet and a healthy lifestyle.